Evolving strategies for enzyme engineering.

نویسندگان

  • Jesse D Bloom
  • Michelle M Meyer
  • Peter Meinhold
  • Christopher R Otey
  • Derek MacMillan
  • Frances H Arnold
چکیده

Directed evolution is a common technique to engineer enzymes for a diverse set of applications. Structural information and an understanding of how proteins respond to mutation and recombination are being used to develop improved directed evolution strategies by increasing the probability that mutant sequences have the desired properties. Strategies that target mutagenesis to particular regions of a protein or use recombination to introduce large sequence changes can complement full-gene random mutagenesis and pave the way to achieving ever more ambitious enzyme engineering goals.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Potentials of Evolving Linear Models in Tracking Control Design for Nonlinear Variable Structure Systems

Evolving models have found applications in many real world systems. In this paper, potentials of the Evolving Linear Models (ELMs) in tracking control design for nonlinear variable structure systems are introduced. At first, an ELM is introduced as a dynamic single input, single output (SISO) linear model whose parameters as well as dynamic orders of input and output signals can change through ...

متن کامل

Engineering enzymes for biocatalysis.

Protein engineering techniques have been available for over two decades beginning with the development of methods for genetic engineering. Since that time, the engineering of enzymes has advanced rapidly along with a revolution in the range and efficiency of new techniques and strategies for designing and evolving proteins in the laboratory. While recent advances in high-throughput screening te...

متن کامل

A Fuzzy Multi Objective Programming Model for Power Generation and Transmission Expansion Planning Problem

The increasing consumption of electricity over time forces different countries to establishnew power plants and transmission lines. There are various crisp single-objective mathematicalmodels in the literature for the long-term power generation and transmission expansion planning tohelp the decision makers to make more reasonable decisions. But, in practice, most of the parametersassociated wit...

متن کامل

Enzyme engineering: rational redesign versus directed evolution.

Enzyme engineering is undergoing the most profound and exciting transformation in its history (1). It promises unprecedented expansion in the scope and applications of modified or improved enzymes with desired physical and catalytic properties. Two complementary strategies are currently available: rational redesign (2-5) and directed evolution (6,7). Although both approaches have met with great...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Current opinion in structural biology

دوره 15 4  شماره 

صفحات  -

تاریخ انتشار 2005